Production of xylose through enzymatic hydrolysis of glucuronoarabinoxylan from brewers’ spent grain

Abstract Xylose is an abundant bioresource for obtaining diverse chemicals and added-value products. The production of xylose from green alternatives like enzymatic hydrolysis important step in a biorefinery context. This research evaluated the synergism among four classes hydrolytic purified enzymes—endo-1,4-?-xylanase, ?- l -arabinofuranosidase, ?-xylosidase, d -glucuronidase—over glucuronoarabinoxylan (GAX) obtained brewers’ spent grain (BSG) after alkaline extraction ethanol precipitation. First, monosaccharides, uronic acids glycosidic-linkages extracted GAX fraction BSG were characterized, that different strategies based on addition one or two families enzymes—endo-1,4-?-xylanase (GH10 GH11) -arabinofuranosidase (GH43 GH51)—cooperating with ?-xylosidase (GH43) -glucuronidase (GH67) into assessed to obtain best yield xylose. release was monitored over time first 90 min prolonged reaction up 48 h reaction. highest 63.6% (48 h, 40 ?, pH 5.5), using mixture all enzymes devoid family. These results highlight importance GH51 arabinofuranosidase debranching enzyme allow higher cleavage xylan backbone their synergy 2 endo-1,4-?-xylanase GH11), inclusion system. Therefore, this study provides environmentally friendly process produce through utilization as catalysts. Graphical